Gibco™ RPMI 1640 培養(yǎng)基（ATCC 改良）相關(guān)介紹：

RPMI 1640 Medium was originally developed for the suspension culture of human leukemic monolayer cells. Roswell Park Memorial Institute (RPMI) 1640 Medium has been found to be suitable for a variety of mammalian cells including HeLa cells, Jurkat cells, MCF-7 cells, PC12 cells, PBMC cells, astrocytes, and cancer cells. For a wide range of cell culture applications, we offer RPMI 1640 Modified Medium in various components. Use the media selection tool to find suitable formulations.

This RPMI is modified as follows:

Include

L-Glutamine

HEPES:

Phenol red

sodium pyruvate

high sugar

low sodium bicarbonate

Full formulations are available

RPMI 1640 medium is unique compared to other media because it contains the reducing agent glutathione and high concentrations of vitamins. RPMI 1640 medium contains biotin, vitamin B 12 and PABA, which are not available in Eagle's MEM and DMEM. In addition, the medium contains high concentrations of the vitamins inositol and choline. This RPMI formula has been modified by ATCC to contain high concentrations of dextrose, low sodium bicarbonate, sodium pyruvate, HEPES and low L-glutamine. RPMI 1640 Medium does not contain proteins, lipids or growth factors. Therefore, RPMI 1640 medium requires supplements, usually 10% fetal bovine serum (FBS). RPMI 1640 medium uses a sodium bicarbonate buffer system (1.5 g/L), thus requiring an environment of 5–7% CO 2 to maintain physiological pH.

cGMP Manufacturing and Quality System：

RPMI 1640 Medium is manufactured in a cGMP compliant facility located in Grand Island, NY. The facility is an FDA registered medical device manufacturer and is ISO 13485 certified.

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